标题: Characterization and genome analysis of Acinetobacter oleivorans S4 as an efficient hydrocarbon-degrading and plant-growth-promoting rhizobacterium

作者: Wang, PP (Wang, Panpan); Wei, HY (Wei, Haiying); Ke, T (Ke, Tan); Fu, YJ (Fu, Yaojia); Zeng, YY (Zeng, Yuyang); Chen, CQ (Chen, Chaoqi); Chen, LZ (Chen, Lanzhou)

来源出版物: CHEMOSPHERE卷: 331文献号: 138732 DOI: 10.1016/j.chemosphere.2023.138732提前访问日期: MAY 2023出版年: AUG 2023

摘要: Plant-growth-promoting rhizobacteria (PGPR) have received increasing attention for assisting phytoremediation. However, the effect of PGPR on total petroleum hydrocarbon (TPH) degradation and plant growth promotion and its underlying mechanism is not well understood. In this study, phenotypic analysis and whole genome sequencing were conducted to comprehensively characterize a newly isolated rhizobacterium strain S4, which was identified as Acinetobacter oleivorans, from a TPH-contaminated soil. The strain degraded 62.5% of initially spiked diesel (1%) in minimal media within six days and utilized n-alkanes with a wide range of chain length (i.e., C12 to C40). In addition, the strain showed phenotypic traits beneficial to plant growth, including siderophore production, indole-3-acetic acid synthesis and phosphate solubilization. Potential metabolic pathways and genes encoding proteins responsible for the phenotypic traits were identified. In a real TPH-contaminated soil, inoculation of Acinetobacter oleivorans S4 significantly enhanced the growth of tall fescue relative to the soil without inoculation. In contrast, inoculation of Bacillus sp. Z7, a hydrocarbon-degrading strain, showed a negligible effect on the growth of tall fescue. The removal efficiency of TPH with inoculation of Acinetobacter oleivorans S4 was significantly higher than those without inoculation or inoculation of Bacillus sp. Z7. These results suggested that traits of PGPR beneficial to plant growth are critical to assist phytoremediation. Furthermore, heavy metal resistance genes and benzoate and phenol degradation genes were found in the genome of Acinetobacter oleivorans S4, suggesting its application potential in broad scenarios.

作者关键词: Total petroleum hydrocarbons; Biodegradation; Plant growth-promoting rhizobacteria; Whole genome sequencing

地址: [Wang, Panpan; Wei, Haiying; Ke, Tan; Fu, Yaojia; Zeng, Yuyang; Chen, Chaoqi; Chen, Lanzhou] Wuhan Univ, Hubei Res Ctr Environm Remediat Technol, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Wuhan 430079, Peoples R China.

通讯作者地址: Chen, CQ; Chen, LZ (通讯作者)，Wuhan Univ, Hubei Res Ctr Environm Remediat Technol, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Wuhan 430079, Peoples R China.

电子邮件地址: chenchaoqi@whu.edu.cn;chenlz@whu.edu.cn

影响因子：8.8