标题: Mechanism of the inhibition and detoxification effects of the interaction between nanoplastics and microalgae Chlorella pyrenoidosa

作者: Wenfeng Yang, Pan Gao, Huixiang Li, Jiayi Huang, Yan Zhang, Huijun Ding, Weihao Zhang

来源出版物: SCIENCE OF THE TOTAL ENVIRONMENT卷: 783文献号: 146919 DOI: 10.1016/j.scitotenv.2021.146919出版年: Apr 06 2021

摘要: Most previous studies have focused on the toxicity of microplastics on aquatic organisms. However, research on nanoplastics is still limited and poses significant threat to aquatic organisms than microplastics. Therefore, this study investigated the effects of nanoplastics (80 nm) on the microalgae Chlorella pyrenoidosa. One unanticipated finding was that inhibition and detoxification effects existed in the interaction between nanoplastics and C. pyrenoidosa. Nanoplastics contributed the maximum inhibition rates of 27.73%, 29.64%, and 11.76% on algal growth, chlorophyll a, and Fv/Fm, respectively, which were much higher than those of microplastics. However, the inhibitory effect of nanoplastics gradually decreased with prolonged exposure time after reaching a maximum. The transcriptomic analysis explained that the inhibition effect of nanoplastics was due to the blockage of the gene expression of aminoacyl tRNA synthetase and the synthesis of related enzymes and proteins at low concentrations (10 mg·L−1). Moreover, it affected DNA damage repair and hindered photosynthesis at high concentrations (50 mg·L−1). The detoxification phenomenon is attributed to the promotion of cell proliferation, the acceleration of the degradation of damaged proteins and organs, and the regulation of intracellular osmotic pressure in algae. The results of this study provide an understanding of the mechanism underlying the interaction between nanoplastics and microalgaed.

PubMed ID:33866172

语言: English

文献类型: Article

作者关键词: nanoplastics

KeyWords Nanoplastics; Microalgae; Gene expression; Inhibition; Detoxification

地址: [Wenfeng Yang, Huixiang Li, Jiayi Huang, Yan Zhang, Weihao Zhang] Wuhan Univ, Sch Resource & Environm Sci, 129 Luoyu Rd, Wuhan 430079, Peoples R China.

[Pan Gao] Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

[Huijun Ding] Jiangxi Provincial Key Laboratory of Water Resources and Environment of Poyang Lake, Jiangxi Institute of Water Sciences, Nanchang, Jiangxi 330029, PR China.

通讯作者地址: weihao. zhang (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, 129 Luoyu Rd, Wuhan 430079, Peoples R China.

电子邮件地址:weihao.zhang@outlook.com.

标题: Comparison of the effects of continuous and accumulative exposure to nanoplastics on microalga Chlorella pyrenoidosa during chronic toxicity

作者: Wenfeng Yang, Pan Gao, Ye Nie Jiayi Huang, Yixiao Wu, Liang Wan, Huijun Ding, Weihao Zhang

来源出版物: SCIENCE OF THE TOTAL ENVIRONMENT卷: 788文献号: 147934

DOI: 10.1016/j.scitotenv.2021.147934出版年:May 21 2021

摘要: Most previous studies have focused on the continuous exposure of aquatic organisms to nanoplastics. However, persistent pollutants in natural aquatic surroundings are a threat, and their concentrations are continuously increasing. The discussion and research into the effects of accumulative exposure to these materials are limited. Therefore, this study aimed to compare the effects of continuous and accumulative exposure to polystyrene (PS) nanoplastics (80 nm) on Chlorella pyrenoidosa during chronic toxicity. The results indicated that under conditions of continuous exposure, this alga exhibited self-recovery to defend against the negative effects of PS nanoplastics during 15–21 days of exposure (the 21-d inhibitory rate was 1.41%). However, one unanticipated finding was that during the same period of accumulative exposure, nanoplastics retained a substantial and stable inhibitory effect on the algal growth (the 21-d inhibitory rate was 6.79% in accumulative exposure for twice), indicating the invalid self-recovery of algae. The results of scanning electron microscopy demonstrated that on day 21, the degree of damage to the algal cells under accumulative exposure was more severe than that under continuous exposure. Hence, nanoplastics exerted an irreversibly negative effect on aquatic organisms depending on the pattern, frequency, concentration, and duration of exposure. This project evaluated the practical significance of nanoplastics in aquatic ecosystems.

语言: English

文献类型: Article

作者关键词: nanoplastics

KeyWords: Microalgae; Chronic toxicity; Continuous exposure;Accumulative exposure

地址: [Wenfeng Yang, Ye Nie, Jiayi Huang, Yixiao Wu, Liang Wan, Weihao Zhang] Wuhan Univ, Sch Resource & Environm Sci, 129 Luoyu Rd, Wuhan 430079, Peoples R China.

[Pan Gao] Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

[Huijun Ding] Jiangxi Provincial Key Laboratory of Water Resources and Environment of Poyang Lake, Jiangxi Institute of Water Sciences, Nanchang, Jiangxi 330029, PR China.

通讯作者地址: weihao. zhang (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, 129 Luoyu Rd, Wuhan 430079, Peoples R China.

电子邮件地址: weihao.zhang@outlook.com.

影响因子：6.551