标题: Accelerating dermal wound healing and mitigating excessive scar formation using LBL modified nanofibrous mats

作者: Wu, GM (Wu, Guomin); Ma, X (Ma, Xiao); Fan, L (Fan, Le); Gao, YY (Gao, Yuying); Deng, HB (Deng, Hongbing); Wang, YN (Wang, Yining)

来源出版物: MATERIALS & DESIGN卷: 185文献号: 108265 DOI: 10.1016/j.matdes.2019.108265出版年: JAN 5 2020

摘要: Developing an ideal wound dressing material which could accelerate wound closure and achieve starless wound healing was the ultimate goal of numerous researchers. In this study, biomimetic silk fibroin (SF)/polycaprolactone (PCL) matrices were fabricated via co-electrospinning and positively charged chitosan (CS) and negatively charged type I collagen (COL) were deposited on the nanofibrous mats through electrostatic layer-by-layer (LBL) self-assembly technique. Scanning electron microscopy images indicate that the average fiber diameter of SF/PCL nanofibers became larger and more and more irregular protuberances were observed on their surfaces with the LBL process. Besides, the chemical structure and composition investigation further verified the successful deposition of CS/COL. Additionally, tensile strength and water contact angle tests showed the LBL modified mats had enhanced mechanical properties and good hydrophilicity. Moreover, LBL structured mats acquired excellent antibacterial activity and better ability to promote cell attachment, growth and proliferation. Ultimately, in vivo wound healing assay in rat models revealed that LBL structured mats could reduce the wound closure time, increase collagen production and mitigate excessive scar formation through TGF-beta/Smad signaling pathways, which demonstrated the potential application of the nanofibrous mats in skin regeneration. (C) 2019 Published by Elsevier Ltd.

入藏号: WOS:000504639400003

语言: English

文献类型: Article

作者关键词: Silk fibroin; Chitosan; Collagen; Nanofibrous mats; Wound healing

地址: [Wu, Guomin; Fan, Le; Wang, Yining] Wuhan Univ, Sch & Hosp Stomatol, State Key Lab Breeding Base Basic Sci Stomatol Hu, Minist Educ, Wuhan 430079, Hubei, Peoples R China.

[Wu, Guomin; Fan, Le; Wang, Yining] Wuhan Univ, Sch & Hosp Stomatol, Minist Educ, Key Lab Oral Biomed, Wuhan 430079, Hubei, Peoples R China.

[Wu, Guomin; Gao, Yuying; Deng, Hongbing] Wuhan Univ, Sch Resource & Environm Sci, Hubei Int Sci & Technol Cooperat Base Sustainable, Hubei Key Lab Biomass Resource Chem & Environm Bi, Wuhan 430079, Hubei, Peoples R China.

[Ma, Xiao] Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Guangzhou 510080, Guangdong, Peoples R China.

通讯作者地址: Wang, YN (通讯作者)，Wuhan Univ, Sch & Hosp Stomatol, State Key Lab Breeding Base Basic Sci Stomatol Hu, Minist Educ, Wuhan 430079, Hubei, Peoples R China.

Wang, YN (通讯作者)，Wuhan Univ, Sch & Hosp Stomatol, Minist Educ, Key Lab Oral Biomed, Wuhan 430079, Hubei, Peoples R China.

Deng, HB (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Int Sci & Technol Cooperat Base Sustainable, Hubei Key Lab Biomass Resource Chem & Environm Bi, Wuhan 430079, Hubei, Peoples R China.

电子邮件地址: hbdeng@whu.edu.cn;wang.yn@whu.edu.cn

影响因子：5.77